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The origin of dna replication of bacteriophage f1 functions as a signal not only for initiation of viral strand synthesis but also for its termination.
F1 origin of replication. The origin of replication also called the replication origin is a particular sequence in a genome at which replication is initiated. Phage f1 is a filamentous rod shaped ssdna phage with a molecular mass of about 16 10 7 da. Addgenes blog post. Propagation of the genetic material between generations requires timely and accurate duplication of dna by semiconservative replication prior to cell division to ensure each daughter cell receives the full complement of chromosomes.
The flexible phages are about 850 nm long and 43 or 63 nm wide. The ends of the filament are sealed with protein caps. Viral plus strand synthesis initiates and terminates at a specific site plus origin that is recognized and nicked by the viral gene ii protein. The thousands of identical major coat proteins that make up the sheath are arranged in a fishcale like a helix.
By weight it is 113 percent dna. It can be used as a type of cloning vector in combination with filamentous phage m13. The blunt end. Mutational analysis of the 5 side upstream of the origin of plus strand replication of phage f1 led us to postulate the existence of a set of overlapping functional domains.
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